Chapter 7 Methods Ex vivo measurements on freshly resected human colonic specimens were performed in the operating theatres of Maastricht University Medical Center (MUMC, Maastricht, The Netherlands). Material The equipment used for these ex vivo measurements was installed on a modified ‘goLab Mobile Work Station’ (see schematic set‐up in Figure 7.1), which consisted of: a notebook, a transformer, a spectrometer, a light source and a non‐sterile fiber probe. The ‘Medical Technology Service Department’ tested and approved the equipment according to the essential requirements of IEC 60601‐1 to assure the patient’s safety. The equipment was centrally driven by a computer. To facilitate the mobility of the equipment, a notebook (HP Pavilion dv6, installed with Windows® 7 Home Premium) was chosen instead of a desktop. The spectra recorded were directly saved on the notebook hard disk for further analysis. Figure 7.1 Schematic set‐up equipment A medical isolating transformer (KLMX/S‐1200‐230/230, Muuntosähkö Oy/Trafox, Helsinki Finland) was used to connect the different electrical components to mains power thereby ensuring that leakage currents were limited to values permitted for medical equipment8. 92
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